USP L26 column: C4 (butyl silane)
Butyl (C4) silane bonded to fully or superficially porous silica of 1.5-10 um.
Mode: reversed-phase Typical pH range: about 2-8 (silica base)
Typical uses
- Peptides and proteins (wide-pore versions)
- Very hydrophobic small molecules
Common sizes (length x internal diameter, particle size)
- 250 x 4.6 mm, 5 um
- 150 x 4.6 mm, 5 um
- 100 x 2.1 mm, 1.7-1.8 um
For proteins choose wide-pore (about 300 A) material.
Tender wording (brand-neutral)
HPLC column with USP L26 packing (C4 (butyl silane)), 250 x 4.6 mm, 5 um (length x internal diameter, particle size); stainless-steel or equivalent hardware with standard fittings; recommended pH and temperature range to be stated by the bidder; each column supplied with its certificate of analysis and test chromatogram, batch/lot number and serial number; shelf life not less than 2 years from delivery; any make meeting the USP L26 designation and the stated dimensions is acceptable.
Size:
Changing length or particle size
You can keep the USP L26 packing and use a different length, particle size or diameter within the harmonized USP 621 limits. Check your columns with the calculator.
Related column types
L1 C18 (octadecyl silane) | L7 C8 (octyl silane) | L10 Cyano (CN, nitrile) | L11 Phenyl | L13 C1 (trimethylsilane) | L21 PS-DVB polymer (rigid) | L29 Polybutadiene-coated alumina (RP) | L43 PFP (pentafluorophenyl) | L60 Polar-embedded alkyl amide, endcapped | L62 C30 | L67 Polymer C18 (vinyl alcohol copolymer) | L68 Alkyl amide, not endcapped
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