USP L25 column: Polymethacrylate SEC, water-soluble polymers (MW ~100-5000)
Size-exclusion packing on cross-linked polymethacrylate resin able to separate water-soluble neutral, anionic and cationic polymers of about 100-5000 Da (polyethylene oxide calibration).
Mode: size-exclusion Typical pH range: wide, roughly 1-13 (polymer base)
Typical uses
- Molecular weight of PEGs and low-MW polymers
- Oligosaccharides and small polymer excipients
Common sizes (length x internal diameter, particle size)
- 300 x 7.8 mm, 5-8 um
- 300 x 7.5 mm, 10-13 um
Tender wording (brand-neutral)
HPLC column with USP L25 packing (Polymethacrylate SEC, water-soluble polymers (MW ~100-5000)), 300 x 7.8 mm, 5-8 um (length x internal diameter, particle size); stainless-steel or equivalent hardware with standard fittings; recommended pH and temperature range to be stated by the bidder; each column supplied with its certificate of analysis and test chromatogram, batch/lot number and serial number; shelf life not less than 2 years from delivery; any make meeting the USP L25 designation and the stated dimensions is acceptable.
Size:
Changing length or particle size
You can keep the USP L25 packing and use a different length, particle size or diameter within the harmonized USP 621 limits. Check your columns with the calculator.
Related column types
L33 SEC silica for dextrans (4-500 kDa) | L39 Hydrophilic polyhydroxymethacrylate gel (SEC) | L59 Protein SEC on coated silica/hybrid (5-7000 kDa)
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