USP L59 column: Protein SEC on coated silica/hybrid (5-7000 kDa)
Size-exclusion packing for proteins of roughly 5-7000 kDa: spherical silica or hybrid particles of 1.5-10 um with a hydrophilic coating.
Mode: size-exclusion Typical pH range: about 2.5-7.5 (silica); wider for hybrid
Typical uses
- Aggregates, dimers and fragments of insulin, heparin-type and biologic products
- Molecular size distribution of proteins
Common sizes (length x internal diameter, particle size)
- 300 x 7.8 mm, 5 um
- 150 x 4.6 mm, 1.7-3 um
Pore size must suit the protein; L59 covers many pore sizes.
Tender wording (brand-neutral)
HPLC column with USP L59 packing (Protein SEC on coated silica/hybrid (5-7000 kDa)), 300 x 7.8 mm, 5 um (length x internal diameter, particle size); stainless-steel or equivalent hardware with standard fittings; recommended pH and temperature range to be stated by the bidder; each column supplied with its certificate of analysis and test chromatogram, batch/lot number and serial number; shelf life not less than 2 years from delivery; any make meeting the USP L59 designation and the stated dimensions is acceptable.
Size:
Changing length or particle size
You can keep the USP L59 packing and use a different length, particle size or diameter within the harmonized USP 621 limits. Check your columns with the calculator.
Related column types
L25 Polymethacrylate SEC, water-soluble polymers (MW ~100-5000) | L33 SEC silica for dextrans (4-500 kDa) | L39 Hydrophilic polyhydroxymethacrylate gel (SEC)
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