USP L27 column: Silica, porous (30-50 um)
Porous silica particles of 30-50 um.
Mode: normal-phase Typical pH range: about 2-8 (silica base)
Typical uses
- Preparative / flash-type separations
- Older large-particle methods
Common sizes (length x internal diameter, particle size)
- short guard / pre-column, dry-packed 30-50 um
Large particles; not for modern analytical HPLC.
Tender wording (brand-neutral)
HPLC column with USP L27 packing (Silica, porous (30-50 um)), short guard / pre-column, dry-packed 30-50 um (length x internal diameter, particle size); stainless-steel or equivalent hardware with standard fittings; recommended pH and temperature range to be stated by the bidder; each column supplied with its certificate of analysis and test chromatogram, batch/lot number and serial number; shelf life not less than 2 years from delivery; any make meeting the USP L27 designation and the stated dimensions is acceptable.
Changing length or particle size
You can keep the USP L27 packing and use a different length, particle size or diameter within the harmonized USP 621 limits. Check your columns with the calculator.
Related column types
L3 Bare silica | L8 Amino (aminopropyl silane) | L20 Diol
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