USP L56 column: C3 (propyl silane)

Propyl (C3) silane bonded to fully or superficially porous silica of 3-10 um.

Mode: reversed-phase   Typical pH range: about 2-8 (silica base)

Typical uses

  • Proteins and large peptides (wide-pore)
  • Weakly retained reversed-phase separations

Common sizes (length x internal diameter, particle size)

  • 250 x 4.6 mm, 5 um
  • 150 x 4.6 mm, 3-5 um

Tender wording (brand-neutral)

HPLC column with USP L56 packing (C3 (propyl silane)), 250 x 4.6 mm, 5 um (length x internal diameter, particle size); stainless-steel or equivalent hardware with standard fittings; recommended pH and temperature range to be stated by the bidder; each column supplied with its certificate of analysis and test chromatogram, batch/lot number and serial number; shelf life not less than 2 years from delivery; any make meeting the USP L56 designation and the stated dimensions is acceptable.

Size:

Changing length or particle size

You can keep the USP L56 packing and use a different length, particle size or diameter within the harmonized USP 621 limits. Check your columns with the calculator.

Related column types

L1 C18 (octadecyl silane) | L7 C8 (octyl silane) | L10 Cyano (CN, nitrile) | L11 Phenyl | L13 C1 (trimethylsilane) | L21 PS-DVB polymer (rigid) | L26 C4 (butyl silane) | L29 Polybutadiene-coated alumina (RP) | L43 PFP (pentafluorophenyl) | L60 Polar-embedded alkyl amide, endcapped | L62 C30 | L67 Polymer C18 (vinyl alcohol copolymer)

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