USP L36 column: Pirkle chiral (DNB-phenylglycine)

3,5-Dinitrobenzoyl derivative of L-phenylglycine covalently bonded to 5 um aminopropyl silica.

Mode: chiral   Typical pH range: about 2-9 in RP mode; mainly used with organic/normal-phase eluents

Typical uses

  • Normal-phase enantiomer separations of pi-basic compounds

Common sizes (length x internal diameter, particle size)

  • 250 x 4.6 mm, 5 um
  • 150 x 4.6 mm, 3 um

Tender wording (brand-neutral)

HPLC column with USP L36 packing (Pirkle chiral (DNB-phenylglycine)), 250 x 4.6 mm, 5 um (length x internal diameter, particle size); stainless-steel or equivalent hardware with standard fittings; recommended pH and temperature range to be stated by the bidder; each column supplied with its certificate of analysis and test chromatogram, batch/lot number and serial number; shelf life not less than 2 years from delivery; any make meeting the USP L36 designation and the stated dimensions is acceptable.

Size:

Changing length or particle size

You can keep the USP L36 packing and use a different length, particle size or diameter within the harmonized USP 621 limits. Check your columns with the calculator.

Related column types

L32 Chiral ligand exchange (L-proline copper) | L40 Cellulose tris(3,5-dimethylphenylcarbamate), coated | L41 Alpha1-acid glycoprotein (AGP) chiral | L45 Beta-cyclodextrin (hydroxypropyl) chiral | L51 Amylose tris(3,5-dimethylphenylcarbamate), coated | L66 Crown ether chiral, (S)-18-crown-6

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