USP L19 column: SCX resin, Ca2+ form (sugars)
Strong cation-exchange resin of sulfonated, cross-linked styrene-divinylbenzene in the calcium form; particles about 5-15 um.
Mode: ion-exchange Typical pH range: about 5-9 (water eluent)
Typical uses
- Sugars (glucose, fructose, lactulose) with water as eluent
- Sugar alcohols
- Excipient assays (ligand exchange)
Common sizes (length x internal diameter, particle size)
- 300 x 7.8 mm, 8-9 um
- 250 x 4.6 mm, 5 um
Run hot (often 80-85 C) with pure water; never use acids or other salts, which strip the calcium.
Tender wording (brand-neutral)
HPLC column with USP L19 packing (SCX resin, Ca2+ form (sugars)), 300 x 7.8 mm, 8-9 um (length x internal diameter, particle size); stainless-steel or equivalent hardware with standard fittings; recommended pH and temperature range to be stated by the bidder; each column supplied with its certificate of analysis and test chromatogram, batch/lot number and serial number; shelf life not less than 2 years from delivery; any make meeting the USP L19 designation and the stated dimensions is acceptable.
Size:
Changing length or particle size
You can keep the USP L19 packing and use a different length, particle size or diameter within the harmonized USP 621 limits. Check your columns with the calculator.
Related column types
L9 SCX on silica | L14 SAX on silica | L17 SCX resin, H+ form (organic acid / ion-exclusion) | L22 Cation-exchange polystyrene gel (sulfonic) | L23 SAX polymethacrylate/polyacrylate gel | L34 SCX resin, Pb2+ form (sugar alcohols) | L46 SAX, latex-agglomerated PS-DVB (9-11 um) | L52 SCX silica (sulfopropyl / sulfoethyl) | L53 WCX resin, carboxylic/phosphonic grafted EVB-DVB (cations IC) | L58 SCX resin, Na+ form (sugars/oligosaccharides)
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