USP L53 column: WCX resin, carboxylic/phosphonic grafted EVB-DVB (cations IC)
Weak cation-exchange resin: ethylvinylbenzene-divinylbenzene with grafted carboxylic and/or phosphonic acid monomers; 3-15 um.
Mode: ion-exchange Typical pH range: 0-14 for most polymer IC resins (check eluent limits)
Typical uses
- Sodium, potassium, lithium, calcium, magnesium and ammonium by IC
- Counter-ion and amine assays
Common sizes (length x internal diameter, particle size)
- 250 x 4 mm (analytical)
- 250 x 2 mm (microbore)
- 50 x 4 mm guard
Usually methanesulfonic acid eluent with suppressed conductivity.
Tender wording (brand-neutral)
HPLC column with USP L53 packing (WCX resin, carboxylic/phosphonic grafted EVB-DVB (cations IC)), 250 x 4 mm (analytical) (length x internal diameter, particle size); stainless-steel or equivalent hardware with standard fittings; recommended pH and temperature range to be stated by the bidder; each column supplied with its certificate of analysis and test chromatogram, batch/lot number and serial number; shelf life not less than 2 years from delivery; any make meeting the USP L53 designation and the stated dimensions is acceptable.
Size:
Changing length or particle size
You can keep the USP L53 packing and use a different length, particle size or diameter within the harmonized USP 621 limits. Check your columns with the calculator.
Related column types
L9 SCX on silica | L14 SAX on silica | L17 SCX resin, H+ form (organic acid / ion-exclusion) | L19 SCX resin, Ca2+ form (sugars) | L22 Cation-exchange polystyrene gel (sulfonic) | L23 SAX polymethacrylate/polyacrylate gel | L34 SCX resin, Pb2+ form (sugar alcohols) | L46 SAX, latex-agglomerated PS-DVB (9-11 um) | L52 SCX silica (sulfopropyl / sulfoethyl) | L58 SCX resin, Na+ form (sugars/oligosaccharides)
Not sure this is the right column? Use the step-by-step column selector.