USP L22 column: Cation-exchange polystyrene gel (sulfonic)
Cation-exchange resin made of porous polystyrene gel carrying sulfonic acid groups; particles about 5-15 um.
Mode: ion-exchange Typical pH range: about 1-13 (resin)
Typical uses
- Organic acids by ion-exclusion
- Sugars and alcohols
- Amino acid type separations
Common sizes (length x internal diameter, particle size)
- 300 x 7.8 mm, 8-9 um
- 250 x 4.6 mm, 5 um
Example columns sold as USP L22
From the makers' own USP code lists and product pages, checked October 2026. Examples only, not an endorsement. Columns with the same code can still differ in selectivity; if the monograph names a column in its notes, start with that one.
| Maker | Product family | Particle | pH range |
|---|---|---|---|
| Hamilton | PRP-X200 | sulfonated PS-DVB cation exchanger, about 10 um | not stated in source |
| Phenomenex | Rezex ROA-Organic Acid H+ (8%) | sulfonated PS-DVB resin, 8% cross-linked, H+ form | not stated in source |
| Merck (Supelco) | SUPELCOGEL H | sulfonated PS-DVB resin, H+ form | not stated in source |
| Waters | IC-Pak Ion Exclusion | polymer resin, 7 um | not stated in source |
| Macherey-Nagel | NUCLEOGEL SCX | sulfonated polymer cation exchanger | not stated in source |
| Resonac (Shodex) | RSpak DC-613 | sulfonated PS-DVB polymer, 6 um (per Waters listing) | not stated in source |
| Shimadzu | Shim-pack Amino-Na | sulfonated polymer cation exchanger | not stated in source |
7 makers listed, so a tender written by USP code stays open to competition.
Tender wording (brand-neutral)
HPLC column with USP L22 packing (Cation-exchange polystyrene gel (sulfonic)), 300 x 7.8 mm, 8-9 um (length x internal diameter, particle size); stainless-steel or equivalent hardware with standard fittings; recommended pH and temperature range to be stated by the bidder; each column supplied with its certificate of analysis and test chromatogram, batch/lot number and serial number; shelf life not less than 2 years from delivery; any make meeting the USP L22 designation and the stated dimensions is acceptable.
Size:
Changing length or particle size
You can keep the USP L22 packing and use a different length, particle size or diameter within the harmonized USP 621 limits. Check your columns with the calculator.
Related column types
L9 SCX on silica | L14 SAX on silica | L17 SCX resin, H+ form (organic acid / ion-exclusion) | L19 SCX resin, Ca2+ form (sugars) | L23 SAX polymethacrylate/polyacrylate gel | L34 SCX resin, Pb2+ form (sugar alcohols) | L46 SAX, latex-agglomerated PS-DVB (9-11 um) | L52 SCX silica (sulfopropyl / sulfoethyl) | L53 WCX resin, carboxylic/phosphonic grafted EVB-DVB (cations IC) | L58 SCX resin, Na+ form (sugars/oligosaccharides)
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