USP L12 column: SAX, quaternary amine on pellicular core
Strong anion exchanger made by bonding a quaternary amine onto solid silica spherical cores; particles 30-50 um.
Mode: ion-exchange Typical pH range: about 2-8
Typical uses
- Older anion-exchange methods
- Guard packing
Common sizes (length x internal diameter, particle size)
- short guard / pre-column, dry-packed 30-50 um
Obsolete pellicular packing.
Tender wording (brand-neutral)
HPLC column with USP L12 packing (SAX, quaternary amine on pellicular core), short guard / pre-column, dry-packed 30-50 um (length x internal diameter, particle size); stainless-steel or equivalent hardware with standard fittings; recommended pH and temperature range to be stated by the bidder; each column supplied with its certificate of analysis and test chromatogram, batch/lot number and serial number; shelf life not less than 2 years from delivery; any make meeting the USP L12 designation and the stated dimensions is acceptable.
Changing length or particle size
You can keep the USP L12 packing and use a different length, particle size or diameter within the harmonized USP 621 limits. Check your columns with the calculator.
Related column types
L9 SCX on silica | L14 SAX on silica | L17 SCX resin, H+ form (organic acid / ion-exclusion) | L19 SCX resin, Ca2+ form (sugars) | L22 Cation-exchange polystyrene gel (sulfonic) | L23 SAX polymethacrylate/polyacrylate gel | L34 SCX resin, Pb2+ form (sugar alcohols) | L46 SAX, latex-agglomerated PS-DVB (9-11 um) | L52 SCX silica (sulfopropyl / sulfoethyl) | L53 WCX resin, carboxylic/phosphonic grafted EVB-DVB (cations IC) | L58 SCX resin, Na+ form (sugars/oligosaccharides)
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