USP L65 column: Strong-acid cation resin, H+ form (coarse)
Strongly acidic cation-exchange resin of sulfonated, cross-linked styrene-divinylbenzene in the hydrogen form; coarse beads (tens to a few hundred um).
Mode: ion-exchange Typical pH range: 0-14 (resin)
Typical uses
- Low-pressure column clean-up / sample preparation
- Classical ion-exchange steps in monographs
Common sizes (length x internal diameter, particle size)
- glass or open column, gravity or low pressure
Not an HPLC packing. Cross-linking and size range quoted differently in sources; check current USP text.
Tender wording (brand-neutral)
HPLC column with USP L65 packing (Strong-acid cation resin, H+ form (coarse)), glass or open column, gravity or low pressure (length x internal diameter, particle size); stainless-steel or equivalent hardware with standard fittings; recommended pH and temperature range to be stated by the bidder; each column supplied with its certificate of analysis and test chromatogram, batch/lot number and serial number; shelf life not less than 2 years from delivery; any make meeting the USP L65 designation and the stated dimensions is acceptable.
Changing length or particle size
You can keep the USP L65 packing and use a different length, particle size or diameter within the harmonized USP 621 limits. Check your columns with the calculator.
Related column types
L9 SCX on silica | L14 SAX on silica | L17 SCX resin, H+ form (organic acid / ion-exclusion) | L19 SCX resin, Ca2+ form (sugars) | L22 Cation-exchange polystyrene gel (sulfonic) | L23 SAX polymethacrylate/polyacrylate gel | L34 SCX resin, Pb2+ form (sugar alcohols) | L46 SAX, latex-agglomerated PS-DVB (9-11 um) | L52 SCX silica (sulfopropyl / sulfoethyl) | L53 WCX resin, carboxylic/phosphonic grafted EVB-DVB (cations IC) | L58 SCX resin, Na+ form (sugars/oligosaccharides)
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